You can completely disable autoplay on Vista computers if needed.
This article discusses a new Group Policy setting which solves the Load Balancing issues with the domain controllers.
Making sure a second hard drive is indexed in Vista requires two settings.
User Account Control is one of the premier security features for Windows Vista client systems. There may be some cases where you need it disabled. You can do this with group policy as outlined here.
If you are troubleshooting a client computer which always communicates to a domain controller in different site, you should look at the two registry entries explained in this article. This article applies to Windows 2000 and Windows XP.